DEPARTMENT OF CRYOENDOCRINOLOGY

RESEARCH DIRECTIONS

The Department focuses on developing advanced approaches to the cryopreservation of multicellular systems, including multicellular spheroids; investigating the effective transplantation of native and cryopreserved endocrine gland cells in animal models of endocrine disorders; generating tissue‑specific stem and progenitor cells of endocrine glands for their subsequent use in cultivation and transplantation; and obtaining neural crest–derived cells in spheroid shape under 3D culture conditions, followed by their cryopreservation and the study of secretome‑mediated effects on neuroendocrine dysfunction.

CURRENT RESEARCH

• Properties of cryopreserved primary endocrine cell cultures from neonatal animals in vitro and in vivo following transplantation
• Morphofunctional characteristics, cryopreservation strategies, and therapeutic potential of 2D and 3D cell cultures derived from neural crest lineages

DEPARTMENT STAFF

Galyna BOZHOK, Dr.Sc. (Biology), Leading Researcher https://orcid.org/0000-0002-4188-9286

Tetyana BONDARENKO, Prof., Dr.Sc. (Biology), Chief Researcher https://orcid.org/0000-0002-5258-3741

Oleksandr PAKHOMOV, PhD (Biology), Senior Researcher https://orcid.org/0000-0002-7494-654X

Olga SYDORENKO, PhD (Biology), Senior Researcher https://orcid.org/0000-0002-1340-7235

Yuliya BOZHKOVA, PhD (Biology), Junior Researcher https://orcid.org/0009-0001-6169-1146

Natalia ALABEDALKARIM, PhD (Biology), Senior Researcher https://orcid.org/0000-0002-4445-9230

Viktoriia KIROSHKA, PhD (Biology), Senior Researcher https://orcid.org/0000-0003-4986-6924

Natalyia KOMAROMI https://orcid.org/0000-0002-9888-0530

Oksana NOVIKOVA https://orcid.org/0000-0003-0046-625X

Anna KAVERINSKA, 2nd category engineer

Olena TSYMBAL, laboratory assistant

MAIN PUBLICATIONS:
>>learn more

• Kiroshka VV, PetrovaVA, Chernyakov DD. Influence of chitosan-chitin nanofiber composites on cytoskeleton structure and the proliferation of rat bone marrow stromal cells. J Mater Sci Mater Med. 2017;28(1):21. https://doi.org/10.1007/s10856-016-5822-2
• Deng B, Bondarenko T, Pakhomov O. Changes in Sexual Behavior of Orchidectomized Rats Under Influence of Allotransplantation of Testicular Interstitial Cell Suspension. Cell Transplant.  2017;26(5):795–803. https://doi.org/10.3727%2F096368916X693301
• Kiroshka V, Trutaieva I, Bondarenko T. Efficiency of mannitol-supplemented medium during adding/removing ovarian tissue with penetrating cryoprotective agents. Cell&Tissue Banking. 2017;19(1):123-132. https://doi.org/10.1007/s10561-017-9623-8
• Kiroshka VV, Savvova OV, Bozhkova YuO. Spreading and proliferation of cultured rat bone marrow stromal cells on the surface of bioactive glass ceramics. Biopolymers and Cell.  2017;33(1):48–57. doi: http://dx.doi.org/10.7124/bc.00093F 
• Plaksina EM, Sidorenko OS, Bozhok GA. Cryopreservation of Multicellular Spheroids Derived From Newborn Piglet Adrenal Glands. Problems of Cryobiology and Cryomedicine. 2017;27(4):322–333. https://doi.org/10.15407/cryo27.04.322 
• Kiroshka VV, Bozhkova YuO, Trutaieva IA, Gawas AA. Dependence of the Endocrine Function of Ischemic Ovarian Tissue Transplant on the Lipid Peroxidation Intensity International Journal of Physiology and Pathophysiology. 2018;9(1):47–57. https://doi.org/10.1615/IntJPhysPathophys.v9.i1.60
• Ali SG., Bozhok GA. Cryopreservation of Multicellular Spheroids Derived from Primary Culture of Newborn Piglet Spinal Ganglion Cells. Problems of Cryobiology and Cryomedicine. 2019; 29(4):354–364. https://doi.org/10.15407/cryo29.04.354 
• Bohuslavskyi KI, Alabedalkarim NM, Gvozdyuk YaV, Padalko VI, Remnyova NO, Protsenko OS, Bozhok GA. Heterotopic Xenotransplation of Newborn Piglet Aortic FragmentsAfter Hypothermal Incubation in Preservation Solutions. Problems of Cryobiology and Cryomedicine. 2019; 29(1):73–88. https://doi.org/10.15407/cryo29.01.073 
• Kiroshka VV, Borodaі KO, Trutaіeva IA, Pasieshvili NM, Bondarenko TP. Function of Ovarian Tissue Grafts After Hypothermic Storage: Importance of Incubation Medium Composition. Problems of Cryobiology and Cryomedicine. 2019;29(1):19–27.  https://doi.org/10.15407/cryo29.01.019 
• Novikova OYu, Sidorenko OS, Bozhok GA, Bondarenko TP. Cryopreservation Eff ect on Chromogranin A Expression in Neonatal Pig Adrenal Cell Culture. Problems of Cryobiology and Cryomedicine. 2019; 29(4):344–353. https://doi.org/10.15407/cryo29.04.344 
• Globa VYu, Bondarenko TP, Bozhok GA, Samburg YYu, Legach EI. Biologically Active Compositions Containing Neurotrophic Factors Change the Contractile Activity of Detrusor of Rats with Infravesical Obstruction. Problems of Cryobiology and Cryomedicine. 2020;30(2):188–198. https://doi.org/10.15407/cryo30.02.188 
• Deng B, Kireev VO, Melentyeva KV, Kovalenko IF, Pakhomov OV. Histology of Cryopreserved Neonatal Rat Testes After Intratesticular Allotransplantation. Problems of Cryobiology and Cryomedicine.  2020;30(1):34–46. https://doi.org/10.15407/cryo30.01.034 
• Ali S, Moiseieva N, Bozhok G.  Cryopreservation of Cell Culture Derived from Dorsal Root Ganglia of Neonatal Pigs. Problems of Cryobiology and Cryomedicine. 2020; 30(2):158–168. https://doi.org/10.15407/cryo30.02.158 
• Globa V, Bondarenko T, Bozhok G, Samburg Y, Legach E. Biologically Active Compositions Containing Neurotrophic Factors Change the Contractile Activity of Detrusor of Rats with Infravesical Obstruction. Problems of Cryobiology and Cryomedicine,  2020;30(2):188–198. https://doi.org/10.15407/cryo30.02.188 
• Deng B, Pakhomov OV, Bozhok GA. Long-term effects of acute cadmium exposure on testis immune privilege. Regulatory Mechanisms in Biosystems, 2020;11(2):180–185. https://doi.org/10.15421/022027 
• Globa VY, Bozhok GA, Legach EI Morphological Parameters of Bladder Rats with Infravesical Obstruction with the Introduction of Biologically Active Compositions. Journal of Pharmacy and Pharmacology. 2020;8:327–333.
• Nesteruk HV, Ustichenko VD, Alabedalkarim NМ, Padalko VI, Protsenko OS, Legach IeI. Impact of Dorsal Root Ganglia Cryoextract on Histological Steatures in Dices and Contractility of Uterus in Differently Aged Rats. Problems of Cryobiology and Cryomedicine. 2021;31(3):258–267. https://doi.org/10.15407/cryo31.03.258 
• Shasha Li, Zhongjie Li, Lingling Fan, Ustichenko VD, Pakhomov OV, Deng Bo. Comparison between slow and rapid freezing for adrenal gland cryopreservation and xenotransplantation. Cryobiology. 2021;102:68–75. https://doi.org/10.1016/j.cryobiol.2021.07.011
• Pakhomov OV. Post-Cryo Survival of Rat Testicular Interstitial Cells in Hydroxyethyl Starch-Based Media.  Problems of Cryobiology and Cryomedicine. 2021;31(1):070–081 https://doi.org/10.15407/cryo31.01.070 
• Pakhomov OV, Sidorenko OS. The impact of cryopreservation with polyvinyl alcohol on the survival and functional activity of rat testis interstitial cells. Biopolymers and Cell. 2021;37(1):14–22. http://dx.doi.org/10.7124/bc.000A48
• Kiroshka VV, Bozhkova YuO, Gavas AA, Rula IA, Bondarenko TP, Semko GO, Pyatikop VO. Endocrine Function of Ovarian Tissue Grafts After Normo- and Hypothermic Storage. Problems of Cryobiology and Cryomedicine, 2021;31(4):326–342. https://doi.org/10.15407/cryo31.04.326 
• Pobielienskyi KO, Pakhomov OV, Gurina TM, Pobielienska LA, Legach IeI., Bozhok GA, Experimental Cryoablation of Thyroid Gland with Propilthiouracyl-Induced Diffuse Hyperplasiae. Problems of Cryobiology and Cryomedicine. 2021;31(2): 168–179. https://doi.org/10.15407/cryo31.02.168 
• Moisieiev AI, Kovalenko IF, Kovalenko SYe, Bozhok GA, Gordiyenko OI. Dynamics of Dimethyl Sulfoxide Penetration Into L929 Cells and L929-Based Spheroids. Probl Cryobiol Cryomed. 2021;31(4):316–325. https://doi.org/10.15407/cryo31.04.316
• Moisieiev AI, Kovalenko IF, Bozhok GA, Gordiyenko OI. Theoretical approaches to determination of optimal cryopreservation regimens for cell spheroids of different cultivation terms Biophysical Bulletin. 2021;46:7-22. https://doi.org/10.26565/2075-3810-2021-46-01

CONTACT PERSON

Galyna BOZHOK
E-mail: G.A.Bozhok@nas.gov.ua