SHARED FACILITY CENTER

The Center was established at the IPCC in February 2024 as a structural unit within the Cryobiochemistry Department of the Institute.  It was created to ensure the most rational use of unique and expensive scientific instruments and equipment produced by both domestic and imported manufacturers, which is impossible or impractical to purchase for each scientific institution separately from centralized funds, joint funds of institutions and organizations, or other sources of funding of the NAS of Ukraine. The main task of the Center is to provide scientists of the NAS of Ukraine with the opportunity to perform studies using  scientific instruments and equipment, which are serviced by qualified personnel capable of maintaining the equipment in high-quality working condition and providing advisory services. The Center  fulfill experimental work in the fields of cryobiology, cryomedicine, cell and molecular biology, cytology, histology, hematology, immunology, microbiology, biotechnology, etc. In addition, the Center provides qualified services to scientists of the institutions of the branches of the National Academy of Sciences of Ukraine, as well as to other scientific and educational institutions in related fields of science, education, medicine, agriculture and the food industry.

AVAILABLE SCIENTIFIC EQUIPMENT

The following high-tech devices are available for use at the Center:

  • Laser scanning confocal microscope LSM 510 META (Carl Zeiss, Germany).
  • Flow cytometer FACS Calibur (Becton Dickinson, USA).
List of main publications, resulted from the findings obtained due to the Center equipment >>>learn more
  • Gordiyenko OI, Kovalenko IF, Rogulska OY, Trufanova NA, Gurina TM, Trufanov OV, Petrenko OY. Theory-based cryopreservation mode of mesenchymal stromal cell spheroids. Cryobiology. 2024. Jun;115:104906. doi: 10.1016/j.cryobiol.2024.104906.
  • Todrin AF, Kovalenko IF, Ye.I. Smolyaninova OV, Timofeyeva LI, Popivnenko, Gordiyenko OI. Determination of cell membrane permeability coefficients: Comparison of models in the case of oocytes. (2023) Cryobiology 113, 104789. https://doi.org/10.1016/j.cryobiol.2023.104789
  • Trufanova N, Rogulska O, Trufanov O, Mazur S, Kovalenko I, Petrenko A. (2023) Cryopreservation of mesenchymal stromal cells spheroids by vitrification
  • Cryobiology, Volume 113, article id. 104751. doi:10.1016/j.cryobiol.2023.104751
  • Chernobai NA, Kadnikova NG, Vozovyk KD, Rozanov LF, Kovalenko, IF. Effects of salt stress and cooling regimes on the pigment composition of Dunaliella salina cells. (2023). Biophysical Bulletin, (49), 34-42. https://doi.org/10.26565/2075-3810-2023-49-03
  • Repina S, Kovalenko I, Nardid O. Temperature dependence of erythrocyte osmotic response to an artificial hypobiosis state of homoiothermal and heterothermal mammals. (2022) Cryobiology 109, 63.
  • Chernobai NA, Kadnikova, N. G., Vozovyk, K. D., Rozanov, L. F., Kovalenko, I. F., & Kot, Y. G. Temperature-salt stress increases yield of valuable metabolites and shelf life of microalgae. (2022). Biophysical Bulletin, (48), 7-17. https://doi.org/10.26565/2075-3810-2022-48-01
  • Smolyaninova Y, Shigimaga V, Popivnenko L, Kovalenko I. Cell-to-Cell Adhesion and Electrical Parameters of Murine Embryo Membranes After Cryopreservation by Vitrification. Problems of Cryobiology and Cryomedicine. (2022) 32 (4), 256–266-256–266.
  • Shevchenko N, Chernobai N, Vozovyk K, Kovalenko I, Rozanov L. Determination of microalgae Chlorococcum dissectum cells permeability coefficient for cryoprotectants. (2022) Cryobiology, 109, 60.
  • Moisieiev A, Kovalenko I, Kovalenko S, Bozhok D, Gordienko O. Dynamics of dimethyl sulfoxide penetration into L929 cells and L929-based spheroids.  Probl.Cryobiol.Cryomed. 2021; 31(4):316–325. https://doi.org/10.15407/cryo31.04.316
  • Moisieiev AI, Kovalenko IF, Bozhok GA, Gordiyenko OI. Theoretical approaches to determination of optimal cryopreservation regimens for cell spheroids of different cultivation terms. 2021/12/29 Biophysical Bulletin Випуск 46 7-22. DOI: https://doi.org/10.26565/2075-3810-2021-46-01
  • Kuleshova L, Kovalenko I, Kovalenko S, Tsibulko T, Gordienko O. Transport characteristics of plasma membranes of PK-15 passaged cells. Probl. Cryobiol. Cryomed. 2021; 31(1):014–022. https://doi.org/10.15407/cryo31.01.014
  • Goltsev АМ, Babenko NM, Gaevska YO, Bondarovich MO, Оstankov MV, Kovalenko IF, Malyukin YV, Klochkov VK. Antitumor efficiency of hybrid nanocomplexes depends on the time of their interaction with ehrlich carcinoma cells. Nanosistemi, Nanomateriali, Nanotehnologii. 2020, т. 18, № 3, pp. 487–503.
  • Klochkov VL, Goltsev AM, Malyukin YV, Babenko NM, Gaevska YO, Bondarovych MO, Dubrava TG, Kovalenko IF, Ostankov MV. Antitumor activity of spherical nanoparticles GdYVO4:Eu3+ depends on pre-incubation time.  Applied Nanoscience. 2020; 10(4). DOI:10.1007/s13204-020-01284-3
  • Deng B, Kireev V, Melentyeva K, Kovalenko I, Pakhomov O. Histology of Cryopreserved Neonatal Rat Testes After Intratesticular Allotransplantation. Problems of Cryobiology and Cryomedicine. 2020; 30(1), pp.34-46. DOI:10.15407/cryo30.01.034
  • Chernobai N, Kadnikova N, Kovalenko I. THE ROLE OF COLD ADAPTATION IN CRYOPRESERVATION OF DUNALIELLA SALINA TEOD. MICROALGAE. Advances in Biology & Earth Sciences Vol.4, No.2, 2019, pp.119-127.
  • Goltsev A, Malyukin Y, Babenko N, Gaevska Y, Bondarovych M, Kovalenko I, Dubrava T, Klochkov V.  Mechanisms of antitumor effect of nanomaterials based on rare earth orthovanadates. Nanooptics and Photonics, Nanochemistry and Nanobiotechnology, and Their Applications: Selected Proceedings of the 7th International Conference Nanotechnology and Nanomaterials (NANO2019), 27–30 August, 2019, Lviv, Ukraine.
  • Ulizko PY, Bobrova OM, Nardid OA, Zubov P, Kovalenko IF, Kuchkov VM, Vodopyanova LA. (2019). Cryopreservation of Equine and Bovine Erythrocytes Using Combined Protective Media. Problems of Cryobiology and Cryomedicine, 29(3), 255–265. https://doi.org/10.15407/cryo29.03.255
  • Kovalenko I, Shckorbatov Y, Miroshnik D. Response to Doxorubicin of Exfoliated Human Buccal Epithelium Cells: Comparison of  Three Methods of Cell Staining and Calcium Assessment. 2018. Current Drug Discovery Technologies, 15, Issue 2, pp.142-148.  DOI: 10.2174/1570163815666171206125949
  • Plaksina KM, Sidorenko OS, Legach YI, Kovalenko IF, Bozhok GA. Expression of  g-III-tubulin in the neonatal adrenal cell culture: comparison of monolayer and 3D-culture. The Journal of VN Karazin Kharkiv National University. Series “Biology”, 2017, issue 28. DOI: 10.26565/2075-5457-2017-28-9
  • Zemlianskykh NG, Kovalenko IF, Babijchuk LA. Peculiarities of Modifications in Geometric Parameters and Changes in Osmotic Fragility of Human Erythrocytes Following Their Exposure in Sucrose and PEG-1500 Solutions.  Probl. Cryobiol. Cryomed. 2017; 27(4):296-310. https://doi.org/10.15407/cryo27.04.296
  • Lytvyn S, Vazhnichaya E, Kurapov Y, Semaka O, Babijchuk L, Zubov P. Cytotoxicity of magnetite nanoparticles deposited in sodium chloride matrix and their functionalized analogues in erythrocytes. OpenNano. 2023; Vol. 11: 100143. https://doi.org/10.1016/j.onano.2023.100143
  • Zubov P, Zubova O, Babijchuk L. Trolox Antioxidant as a Factor in Stabilization of Human Cord Blood Nucleated Cells During Cryopreservation. Problems of Cryobiology and Cryomedicine. (2023). 33(2), 122–132. https://doi.org/10.15407/cryo33.02.122
  • Makashova O, Mykhailova O, Zubova O, Zubov P, Babijchuk L.  Glutathione Antioxidant Increases Resistance of Cord Blood Nucleated Cells During Cryopreservation with Dimethyl Sulfoxide. Problems of Cryobiology and Cryomedicine. (2020). 30(1), 58–67. https://doi.org/10.15407/cryo30.01.058
  • Marchenko IА, Babiichuk LO, Mishyna MM, Makieieva NI, Zubov PM. Peculiarities of leukocyte apoptosis modulation in children with pyelonephritis. Regulatory Mechanisms in Biosystems, (2020). 11(1), 88-92. https://doi.org/10.15421/022012
  • Ulizko P, Bobrova O, Nardid O, Zubov P, Kovalenko I, Kuchkov V, Vodopyanova L. Cryopreservation of Equine and Bovine Erythrocytes Using Combined Protective Media. Probl Cryobiol Cryomed. 2019. V. 29. No. 3, pp. 255-265. https://doi.org/10.15407/cryo29.03.255
  • Onishchenko AI, Tkachenko AS, Kalashnyk JM, Zubov PV, Gorbach TV, Babijchuk LA, Nakonechna OA. Distinguishing modes of leukocyte cell death using flow cytometry in patients with chronic rhinosinusitis without nasal polyps. “MEDICAL JOURNAL” (Serbian Medical Society Section Kragujevac). 2019. Vol. 53, № 2. P. 43-48. doi:10.5937/mckg53-17381
  • Semionova EA, Chabanenko EA, Orlova NV, Zubov PM, NM Shpakova. About Mechanism of Antihemolitic Action of Chlorpromazine Under Posthypertonic Stress in Erythrocytes. Problems of Cryobiology and Cryomedicine. (2017) 27(3), 219–229. https://doi.org/10.15407/cryo27.03.219
  • Makashova OY, Zubova OL, Zubov PM. Role of Antioxidants to Increase Survival and Viability of Cryopreserved Cord Blood Hematopoietic Progenitor Cells. Problems of Cryobiology and Cryomedicine. (2017) 27(2), 176. https://doi.org/10.15407/cryo27.02.176

Contact Person
Volodymyr V. Zeenko, PhD (Biology), Senior Researcher
E-mail: cryo.org.ua@gmail.com

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