Репозитарій відділу кріоендокринології

Study of physical processes occurring in serum-containing and polymer-based serum-free cryoprotective media
- Pakhomov O.V., Gurina T.M., Polyakova H.L., Mazaeva V.S.,Deng Bo, Bozhok G. A.
  - This study aims to investigate the mechanisms of cryoprotection with compositions that were supplemented with polymers (dextran [Dex], hydroxyethyl starch [HES], polyethylene glycol [PEG]) or fetal bovine serum [FBS]). The combinations of dimethyl sulfoxide with FBS or polymers in cryoprotective media were able to promote the formation of amorphous phase and suppress salt eutectic crystallization/melting and recrystallization. The medium supplemented with 0.7 M Me₂SO and 100 mg/ml Dex (0.7Me₂SO+Dex) was somewhat superior compared to other compositions. It also had an optimal glass transition temperature (–26…–21 °C). The feature correlated with an adequate cell recovery when the medium was utilized to preserve testicular cells. The mechanism of cryoprotection depends not only on the concentrations of cryoprotective agents such as Me₂SO, polymers or FBS, but also on the composition of the cryoprotective media. The 0.7Me₂SO+Dex medium can be the basis for the creation of commercial serum-free cryoprotective media with a defined stable composition that does not require removal of Me₂SO before using the cryopreserved cells or tissues for cultivation, transplantation and transfusion.
  - Biopolym. Cell. 2024; 40(1): 37-46. http://dx.doi.org/10.7124/bc.000AAC